Abstract
Tobacco black shank induced by Phytophthora nicotianae causes significant yield losses in tobacco plants. MicroRNAs (miRNAs) play a pivotal role in plant biotic stress responses and have great potential in tobacco breeding for disease resistance. However, the roles of miRNAs in tobacco plants in response to P. nicotianae infection has not been well characterized. In this study, we found that Nta-miR6155, a miRNA specific to Solanaceae crops, was significantly induced in P. nicotianae infected tobacco. Some of predicted target genes of Nta-miR6155 were also observed to be involved in disease resistance. To further investigate the function of miR6155 in tobacco during P. nicotianae infection, Nta-miR6155 overexpression plants (miR6155-OE) were generated in the Honghua Dajinyuan tobacco variety (HD, the main cultivated tobacco variety in China). We found that the Nta-miR6155 overexpression enhanced the resistance in tobacco towards P. nicotianae infections. The level of reactive oxygen species (ROS) was significantly lower and antioxidant enzyme activities were significantly higher in miR6155-OE plants than those in control HD plants during P. nicotianae infection. In addition, we found that the accumulation of salicylic acid and the expression of salicylic acid biosynthesis and signal transduction-related genes is significantly higher in miR6155-OE plants in comparison to the control HD plants. Furthermore, we found that Nta-miR6155 cleaved target genes NtCIPK18 to modulate resistance towards P. nicotianae in tobacco plants. Additionally, phenotypic analysis of miR6155-OE plants showed that Nta-miR6155 could inhibit the growth of tobacco by suppressing nitrogen uptake and photosynthesis. In conclusion, our findings indicated that miR6155 plays a crucial role in the regulation of growth and resistance against P. nicotianae infections in tobacco plants.
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Claims 10
Claim · model-written summary
P. nicotianae infection significantly induced Nta-miR6155 in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Interactor
- P. nicotianae
Phytophthora nicotianaee0pathogen - Gene
- Nta-miR6155e0
- Outcome
- Nta-miR6155e0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nta-miR6155, a miRNA specific to Solanaceae crops, was significantly induced in P. nicotianae infected tobacco”
Claim · model-written summary
Some predicted Nta-miR6155 targets were implicated in disease resistance.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- Nta-miR6155e0
- Outcome
- predicted target genes of Nta-miR6155 were also observed to be involved in disease resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some of predicted target genes of Nta-miR6155 were also observed to be involved in disease resistance”
t0“tobacco”
Claim · model-written summary
Nta-miR6155 overexpression enhanced tobacco resistance to P. nicotianae.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Nta-miR6155 overexpressione0
- Interactor
- P. nicotianaePhytophthora nicotianaee0pathogen
- Gene
- Nta-miR6155e0
- Outcome
- resistance in tobacco towards P. nicotianae infectionse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Nta-miR6155 overexpression enhanced the resistance in tobacco towards P. nicotianae infections”
Claim · model-written summary
Infected miR6155-OE plants had significantly lower ROS than HD controls.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- miR6155-OE plantse0
- Interactor
- P. nicotianaePhytophthora nicotianaee0pathogen
- Outcome
- level of reactive oxygen species (ROS)e0
- Direction
- lowere0
- Comparator
- control HD plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of reactive oxygen species (ROS) was significantly lower and antioxidant enzyme activities were significantly higher in miR6155-OE plants than those in control HD plants during P. nicotianae infection”
t0“tobacco”
Claim · model-written summary
Infected miR6155-OE plants had significantly higher antioxidant enzyme activities than HD controls.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- miR6155-OE plantse0
- Interactor
- P. nicotianaePhytophthora nicotianaee0pathogen
- Outcome
- antioxidant enzyme activitiese0
- Direction
- highere0
- Comparator
- control HD plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of reactive oxygen species (ROS) was significantly lower and antioxidant enzyme activities were significantly higher in miR6155-OE plants than those in control HD plants during P. nicotianae infection”
t0“tobacco”
Claim · model-written summary
miR6155-OE tobacco accumulated significantly more salicylic acid than HD controls.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- miR6155-OE plantse0
- Outcome
- accumulation of salicylic acide0
- Direction
- highere0
- Comparator
- control HD plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation of salicylic acid and the expression of salicylic acid biosynthesis and signal transduction-related genes is significantly higher in miR6155-OE plants in comparison to the control HD plants”
t0“tobacco”
Claim · model-written summary
miR6155-OE tobacco had significantly higher expression of salicylic-acid biosynthesis and signaling genes than HD controls.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- miR6155-OE plantse0
- Outcome
- expression of salicylic acid biosynthesis and signal transduction-related genese0
- Direction
- highere0
- Comparator
- control HD plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation of salicylic acid and the expression of salicylic acid biosynthesis and signal transduction-related genes is significantly higher in miR6155-OE plants in comparison to the control HD plants”
t0“tobacco”
Claim · model-written summary
Nta-miR6155 cleaved NtCIPK18 to modulate resistance against P. nicotianae in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- P. nicotianaePhytophthora nicotianaee0pathogen
- Gene
- NtCIPK18e0
- Outcome
- resistance towards P. nicotianaee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nta-miR6155 cleaved target genes NtCIPK18 to modulate resistance towards P. nicotianae in tobacco plants”
Claim · model-written summary
Nta-miR6155 inhibited tobacco growth by suppressing nitrogen uptake.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- Nta-miR6155e0
- Outcome
- growth of tobaccoe0
- Direction
- inhibite0
- Qualifier
- suppressing nitrogen uptakee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nta-miR6155 could inhibit the growth of tobacco by suppressing nitrogen uptake and photosynthesis”
Claim · model-written summary
Nta-miR6155 inhibited tobacco growth by suppressing photosynthesis.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- Nta-miR6155e0
- Outcome
- growth of tobaccoe0
- Direction
- inhibite0
- Qualifier
- photosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nta-miR6155 could inhibit the growth of tobacco by suppressing nitrogen uptake and photosynthesis”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.