Abstract
Chilling stress has seriously limited the global production and geographical distribution of rice. However, the molecular mechanisms associated with plant responses to chilling stress are less known. In this study, we revealed a member of β-ketoacyl-ACP synthase I family (KASI), OsKASI-2 which confers chilling tolerance in rice. OsKASI-2 encodes a chloroplast-localized KASI enzyme mainly expressed in the leaves and anthers of rice and strongly induced by chilling stress. Disruption of OsKASI-2 led to decreased KAS enzymatic activity and the levels of unsaturated fatty acids, which impairs degree of unsaturation of membrane lipids, thus increased sensitivity to chilling stress in rice. However, the overexpression of OsKASI-2 significantly improved the chilling tolerance ability in rice. In addition, OsKASI-2 may regulate ROS metabolism in response to chilling stress. Natural variation of OsKASI-2 might result in difference in chilling tolerance between indica and japonica accessions, and Hap1 of OsKASI-2 confers chilling tolerance in rice. Taken together, we suggest OsKASI-2 is critical for regulating degree of unsaturation of membrane lipids and ROS accumulation for maintenance of membrane structural homeostasis under chilling stress, and provide a potential target gene for improving chilling tolerance of rice.
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Claims 13
Claim · model-written summary
OsKASI-2 encodes a chloroplast-localized KASI enzyme.
- Taxon
- rice
Oryza sativat0 - Gene
- OsKASI-2e0
- Outcome
- chloroplast-localized KASI enzymee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsKASI-2 encodes a chloroplast-localized KASI enzyme mainly expressed in the leaves and anthers of rice and strongly induced by chilling stress.”
Claim · model-written summary
OsKASI-2 is mainly expressed in rice leaves and anthers.
- Taxon
- riceOryza sativat0
- Organ / stage
- leaves and antherse0
- Gene
- OsKASI-2e0
- Outcome
- mainly expressed in the leaves and antherse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsKASI-2 encodes a chloroplast-localized KASI enzyme mainly expressed in the leaves and anthers of rice and strongly induced by chilling stress.”
Claim · model-written summary
Chilling strongly induces OsKASI-2 expression.
- Taxon
- riceOryza sativat0
- Treatment
- chilling stresse0
- Gene
- OsKASI-2e0
- Outcome
- OsKASI-2e0
- Direction
- strongly inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsKASI-2 encodes a chloroplast-localized KASI enzyme mainly expressed in the leaves and anthers of rice and strongly induced by chilling stress.”
Claim · model-written summary
Disruption of OsKASI-2 decreased KAS enzymatic activity.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of OsKASI-2e0
- Gene
- OsKASI-2e0
- Outcome
- KAS enzymatic activitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Disruption of OsKASI-2 led to decreased KAS enzymatic activity and the levels of unsaturated fatty acids”
Claim · model-written summary
Disruption of OsKASI-2 decreased unsaturated fatty acid levels.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of OsKASI-2e0
- Gene
- OsKASI-2e0
- Outcome
- levels of unsaturated fatty acidse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Disruption of OsKASI-2 led to decreased KAS enzymatic activity and the levels of unsaturated fatty acids”
Claim · model-written summary
Disruption impaired membrane lipid unsaturation.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of OsKASI-2e0
- Gene
- OsKASI-2e0
- Outcome
- degree of unsaturation of membrane lipidse0
- Direction
- impairse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Disruption of OsKASI-2 led to decreased KAS enzymatic activity and the levels of unsaturated fatty acids, which impairs degree of unsaturation of membrane lipids”
Claim · model-written summary
Disruption increased rice sensitivity to chilling stress.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of OsKASI-2e1
- Gene
- OsKASI-2e1
- Outcome
- sensitivity to chilling stresse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“thus increased sensitivity to chilling stress in rice.”
e1“Disruption of OsKASI-2 led to decreased KAS enzymatic activity and the levels of unsaturated fatty acids”
Claim · model-written summary
OsKASI-2 overexpression significantly improved chilling tolerance.
- Taxon
- riceOryza sativat0
- Treatment
- overexpression of OsKASI-2e0
- Gene
- OsKASI-2e0
- Outcome
- chilling tolerance abilitye0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“the overexpression of OsKASI-2 significantly improved the chilling tolerance ability in rice.”
Claim · model-written summary
OsKASI-2 may regulate ROS metabolism during chilling stress.
- Taxon
- riceOryza sativat0
- Gene
- OsKASI-2e0
- Outcome
- ROS metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsKASI-2 may regulate ROS metabolism in response to chilling stress.”
Claim · model-written summary
Natural variation of OsKASI-2 may contribute to chilling tolerance differences between indica and japonica accessions.
- Taxon
- riceOryza sativat0
- Material
- indica and japonica accessionse0
- Gene
- OsKASI-2e0
- Outcome
- difference in chilling tolerancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Natural variation of OsKASI-2 might result in difference in chilling tolerance between indica and japonica accessions”
Claim · model-written summary
OsKASI-2 Hap1 confers chilling tolerance in rice.
- Taxon
- riceOryza sativat0
- Material
- Hap1e0
- Gene
- OsKASI-2e0
- Outcome
- chilling tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Hap1 of OsKASI-2 confers chilling tolerance in rice.”
Claim · model-written summary
The authors suggest OsKASI-2 regulates membrane lipid unsaturation and ROS accumulation under chilling stress.
- Taxon
- riceOryza sativat0
- Gene
- OsKASI-2e0
- Outcome
- degree of unsaturation of membrane lipids and ROS accumulatione0
- Qualifier
- maintenance of membrane structural homeostasis under chilling stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“OsKASI-2 is critical for regulating degree of unsaturation of membrane lipids and ROS accumulation for maintenance of membrane structural homeostasis under chilling stress”
Claim · model-written summary
The authors propose OsKASI-2 as a target for improving rice chilling tolerance.
- Taxon
- riceOryza sativat0
- Gene
- OsKASI-2e1
- Outcome
- improving chilling tolerance of ricee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“provide a potential target gene for improving chilling tolerance of rice”
e1“OsKASI-2 is critical for regulating degree of unsaturation of membrane lipids and ROS accumulation for maintenance of membrane structural homeostasis under chilling stress”
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.