Paper · 2025 · Asian Journal of Soil Science and Plant Nutrition

Effect of Seed Priming, Beejamrit, Jeevamrit and Micronutrients on Symbiotic Traits, Growth Attributes, Dehydrogenase Activity, Leghaemoglobin Content, Protein Content and Grain Yield of Chickpea

S.C. Gupta; Nimisha Maheshwari; Sourabh

12
claims
12
verbatim quotes
0
citations
3
research areas
✓
Open access

Abstract

A field experiment was conducted at Research Farm area of R.A.K. (Rafi Ahmad Kidwai) College of Agriculture, Sehore, Madhya Pradesh during rabi season 2023-24 to study the effect of Seed priming, Beejamrit, Jeevamrit and Micronutrients (Zn and Mo) in combination with Rhizobium and PSB inoculation on symbiotic traits, growth attributes, soil dehydrogenase activity (DHA) and leghaemoglobin content in root nodules, protein content in seed and grain yield of chickpea crop grown with one irrigation in Vertisol. The experiment was laid out in Randomized Block Design (RBD) with 10 treatments and replicated thrice. The treatments comprised of T1: RDF + Rhizobium (Rh) + Phosphate Solubilizing Bacteria (PSB), T2: RDF + Rh + PSB + Seed priming with water, T3: RDF + Rh + PSB + Beejamrit treatment (seed primed), T4: RDF + Rh + PSB + Seed priming with 0.05% Mo as Ammonium Molybdate (AM), T5: RDF + Rh + PSB + AM 1 gm kg-1 seed with inoculation, T6: RDF + Rh + PSB + Jeevamrit spray at 35 and 55 days after sowing (DAS), T7: T1 + 0.05% AM spray at 35 and 55 DAS, T8: T1 + 0.5% ZnSO4 + 0.25% Lime spray at 35 and 55 DAS, T9: T1 + 0.05% AM + 0.25% ZnSO4 + 0.25% Lime spray at 35 and 55 DAS and T10: 50% RDF + Rh + PSB + Jeevamrit spray + 0.05% AM + 0.25% ZnSO4 + 0.25% Lime spray at 35 and 55 DAS. The symbiotic traits, growth attributes at 50 DAS and grain yield was significantly increased by the application of RDF + Rhizobium + PSB + Ammonium Molybdate @ 1 gm kg-1 seed with inoculation (T5), followed by treatment T10 (50% RDF + Rhizobium + PSB + Jeevamrit spray + 0.05% AM + 0.25% ZnSO4 + 0.25% Lime spray at 35 and 55 DAS). The T5 treatment produces about 26% higher grain yield over the RDF + Rhizobium + PSB (T1). The treatment T5 recorded highest protein content in seed i.e. 19.50%, followed by the treatment T10 (19.31%). DHA in rhizosphere soil and leghaemoglobin content in root nodules at 50 DAS was found maximum in treatment T5 (31.20 µg TPF [Triphenyl formazan] g-1 soil hr-1 and 3.10 mg g-1 respectively), followed by treatment T10 with values 30.60 µg TPF g-1 soil hr-1 and 3.08 mg g-1 respectively. The study indicated necessity of application of molybdenum wherever deficient in Vertisol under intensive (Soybean-Chickpea) cultivation of legumes, for enhancing productivity of chickpea and also gave indication that by integration of Jeevamrit spray + 0.05% AM + 0.25% ZnSO4 + 0.25% Lime spray at 35 and 55 DAS, a saving of 50% RDF can be made under existing practice. Further, this study also give an indication that the use of RDF along with biofertilizers i.e. Rhizobium + PSB and seed priming in beejamrit (T3) for four hours before sowing may also be a beneficial option with slightly lower, but statistically identical chickpea yield with highest yielded treatment T5.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 12

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

T5 significantly increased chickpea symbiotic traits and growth attributes.

Treatment
RDF + Rhizobium + PSB + Ammonium Molybdate @ 1 gm kg-1 seed with inoculation (T5)e0
Interactor
RhizobiumRhizobiume0symbiont
Outcome
symbiotic traits, growth attributese0
Direction
increasede0

Not reportedTaxonComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Rhizobium
Rhizobium · Interactor · picture of the genus (one of its species)Ninjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The symbiotic traits, growth attributes at 50 DAS and grain yield was significantly increased by the application of RDF + Rhizobium + PSB + Ammonium Molybdate @ 1 gm kg-1 seed with inoculation (T5), followed by treatment T10”

✓ located verbatim in the source · abstract, characters 1294–1518 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

T5 significantly increased chickpea grain yield.

Treatment
RDF + Rhizobium + PSB + Ammonium Molybdate @ 1 gm kg-1 seed with inoculation (T5)e0
Outcome
grain yielde0
Direction
increasede0

Not reportedTaxonComparator

Verbatim

e0

“The symbiotic traits, growth attributes at 50 DAS and grain yield was significantly increased by the application of RDF + Rhizobium + PSB + Ammonium Molybdate @ 1 gm kg-1 seed with inoculation (T5), followed by treatment T10”

✓ located verbatim in the source · abstract, characters 1294–1518 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T5 produced about 26% more chickpea grain yield than T1.

Treatment
T5 treatmente0
Outcome
grain yielde0
Direction
highere0
Value
26%e0
Comparator
RDF + Rhizobium + PSB (T1)e0

Not reportedTaxon

Verbatim

e0

“The T5 treatment produces about 26% higher grain yield over the RDF + Rhizobium + PSB (T1).”

✓ located verbatim in the source · abstract, characters 1627–1718 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

T5 had the highest chickpea seed protein content at 19.50%.

Treatment
T5e0
Outcome
protein content in seede0
Direction
higheste0
Value
19.50%e0

Not reportedTaxon

Verbatim

e0

“The treatment T5 recorded highest protein content in seed i.e. 19.50%, followed by the treatment T10 (19.31%).”

✓ located verbatim in the source · abstract, characters 1719–1829 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

T10 chickpea seed protein content was 19.31%.

Treatment
T10e0
Outcome
protein content in seede0
Value
19.31%e0

Not reportedTaxon

Verbatim

e0

“The treatment T5 recorded highest protein content in seed i.e. 19.50%, followed by the treatment T10 (19.31%).”

✓ located verbatim in the source · abstract, characters 1719–1829 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

T5 had maximum soil dehydrogenase activity of 31.20 µg TPF g-1 soil hr-1.

Treatment
T5e0
Outcome
DHA in rhizosphere soile0
Direction
maximume0
Value
31.20 µg TPF [Triphenyl formazan] g-1 soil hr-1e0

Not reportedTaxon

Verbatim

e0

“DHA in rhizosphere soil and leghaemoglobin content in root nodules at 50 DAS was found maximum in treatment T5 (31.20 µg TPF [Triphenyl formazan] g-1 soil hr-1 and 3.10 mg g-1 respectively), followed by treatment T10 with values 30.60 µg TPF g-1 soil hr-1 and 3.08 mg g-1 respectively.”

✓ located verbatim in the source · abstract, characters 1830–2115 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

T5 had maximum root nodule leghaemoglobin content of 3.10 mg g-1.

Treatment
T5e0
Outcome
leghaemoglobin content in root nodulese0
Direction
maximume0
Value
3.10 mg g-1e0

Not reportedTaxon

Verbatim

e0

“DHA in rhizosphere soil and leghaemoglobin content in root nodules at 50 DAS was found maximum in treatment T5 (31.20 µg TPF [Triphenyl formazan] g-1 soil hr-1 and 3.10 mg g-1 respectively), followed by treatment T10 with values 30.60 µg TPF g-1 soil hr-1 and 3.08 mg g-1 respectively.”

✓ located verbatim in the source · abstract, characters 1830–2115 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

T10 soil dehydrogenase activity was 30.60 µg TPF g-1 soil hr-1.

Treatment
T10e0
Outcome
DHA in rhizosphere soile0
Value
30.60 µg TPF g-1 soil hr-1e0

Not reportedTaxon

Verbatim

e0

“DHA in rhizosphere soil and leghaemoglobin content in root nodules at 50 DAS was found maximum in treatment T5 (31.20 µg TPF [Triphenyl formazan] g-1 soil hr-1 and 3.10 mg g-1 respectively), followed by treatment T10 with values 30.60 µg TPF g-1 soil hr-1 and 3.08 mg g-1 respectively.”

✓ located verbatim in the source · abstract, characters 1830–2115 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

T10 root nodule leghaemoglobin content was 3.08 mg g-1.

Treatment
T10e0
Outcome
leghaemoglobin content in root nodulese0
Value
3.08 mg g-1e0

Not reportedTaxon

Verbatim

e0

“DHA in rhizosphere soil and leghaemoglobin content in root nodules at 50 DAS was found maximum in treatment T5 (31.20 µg TPF [Triphenyl formazan] g-1 soil hr-1 and 3.10 mg g-1 respectively), followed by treatment T10 with values 30.60 µg TPF g-1 soil hr-1 and 3.08 mg g-1 respectively.”

✓ located verbatim in the source · abstract, characters 1830–2115 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors recommend molybdenum application where Vertisol is deficient for chickpea productivity.

Taxon
chickpeaCicer arietinume0
Outcome
application of molybdenume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The study indicated necessity of application of molybdenum wherever deficient in Vertisol under intensive (Soybean-Chickpea) cultivation of legumes, for enhancing productivity of chickpea”

✓ located verbatim in the source · abstract, characters 2116–2303 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors indicate the integrated Jeevamrit and micronutrient spray can save 50% RDF.

Outcome
saving of 50% RDFe0

Not reportedTaxon

Verbatim

e0

“by integration of Jeevamrit spray + 0.05% AM + 0.25% ZnSO4 + 0.25% Lime spray at 35 and 55 DAS, a saving of 50% RDF can be made under existing practice.”

✓ located verbatim in the source · abstract, characters 2334–2486 · show in text ↑
Intervention effectobservedoriginal resultnegativenot significant (as stated)measured

Claim · model-written summary

Beejamrit seed priming gave a slightly lower but statistically identical yield to T5.

Taxon
chickpeaCicer arietinume0
Treatment
seed priming in beejamrit (T3)e0
Outcome
yielde0
Direction
slightly lowere0
Comparator
treatment T5e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“seed priming in beejamrit (T3) for four hours before sowing may also be a beneficial option with slightly lower, but statistically identical chickpea yield with highest yielded treatment T5.”

✓ located verbatim in the source · abstract, characters 2602–2792 · show in text ↑

Rhizobium · yield · Cicer arietinum (chickpea)Results differ

120 papers: 116 positive, 4 negative or no effect

115 papers in the same direction as this paper · 4 in the opposite direction

  1. no effect
  2. no effect
  3. no effect

116 papers more in this group

See all 120 papers →

Rhizobium · growth · Cicer arietinum (chickpea)Positive and negative results

46 papers: 45 positive, 1 negative or no effect

44 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

42 papers more in this group

See all 46 papers →

ammonium molybdate · growth · Cicer arietinum (chickpea)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

ammonium molybdate · yield · Cicer arietinum (chickpea)Results agree

7 papers: 7 positive, 0 negative or no effect

6 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

3 papers more in this group

See all 7 papers →

phosphorus · growth · Cicer arietinum (chickpea)Results differ

58 papers: 55 positive, 3 negative or no effect

54 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. no effect
  3. negative

54 papers more in this group

See all 58 papers →

seed treatment · yield · Cicer arietinum (chickpea)Results differ

90 papers: 82 positive, 8 negative or no effect

7 papers in the same direction as this paper · 82 in the opposite direction

  1. positive
  2. positive
  3. positive

86 papers more in this group

See all 90 papers →

transgenic · yield · Cicer arietinum (chickpea)Positive and negative results

12 papers: 11 positive, 1 negative or no effect

10 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

8 papers more in this group

See all 12 papers →

fertilization · growth · Cicer arietinum (chickpea)Positive and negative results

105 papers: 99 positive, 6 negative or no effect

98 papers in the same direction as this paper · 6 in the opposite direction

  1. no effect
  2. negative
  3. negative

101 papers more in this group

See all 105 papers →

8 of 13 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.