Abstract
MicroRNAs (miRNAs) are crucial regulators of abiotic stress responses in plants. Among them, miR156 is a highly conserved miRNA family with two mature isoforms: miR156-5p and miR156-3p. While miR156-5p has been extensively studied for its functions in plant growth and stress responses, the roles of miR156-3p remain less explored. In this study, we illustrated that miR156j-3p negatively regulated rice salt tolerance by targeting OsRePRP1.2 . Genetic evidence showed that miR156j-3p downregulation (STTM156j-3p) plants exhibited enhanced salt tolerance. Furthermore, OsRePRP1.2 was confirmed as a direct target of miR156j-3p via 5′ RNA ligase-mediated rapid amplification of cDNA ends (5′-RLM-RACEs) assay in vivo and a rapid transient assay in tobacco. Consistently, OsRePRP1.2 positively regulated rice salt tolerance. Furthermore, both STTM156j-3p and OsRePRP1.2 overexpression ( RePRP1.2 -ox) plants showed enhanced hypersensitivity to exogenous ABA treatment. Besides, physiological assays revealed that the enhanced salt tolerance was associated with reduced accumulation of hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA), along with elevated antioxidant enzyme activities. Finally, we found that the genes involved in ABA signaling, ABA biosynthetic and ROS-scavenging system were up-regulated in STTM156j-3p and RePRP1.2 -ox plants. Taken together, our findings strongly suggest that miR156j-3p targets OsRePRP1.2 and negatively regulates rice salt tolerance through the ROS scavenging and ABA signaling pathways. • miR156j-3p negatively regulates rice salt tolerance by targeting OsRePRP1.2. • miR156j-3p– OsRePRP1.2 module regulates salt tolerance by modulating ROS scavenging. • miR156j-3p– OsRePRP1.2 module regulates salt tolerance by modulating ABA signaling. • Suppression of miR156j-3p or overexpression of OsRePRP1.2 did not affect rice yield.
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Claims 12
Claim · model-written summary
miR156j-3p negatively regulated rice salt tolerance by targeting OsRePRP1.2.
- Taxon
- rice
Oryza sativae0 - Gene
- miR156j-3pe0
- Outcome
- rice salt tolerancee0
- Qualifier
- targeting OsRePRP1.2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR156j-3p negatively regulated rice salt tolerance by targeting OsRePRP1.2”
Claim · model-written summary
Downregulation of miR156j-3p enhanced salt tolerance.
- Taxon
- riceOryza sativat0
- Material
- STTM156j-3pe0
- Treatment
- miR156j-3p downregulatione0
- Outcome
- salt tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR156j-3p downregulation (STTM156j-3p) plants exhibited enhanced salt tolerance.”
t0“rice”
Claim · model-written summary
OsRePRP1.2 was confirmed as a direct target of miR156j-3p.
- Taxon
- riceOryza sativat0
- Gene
- OsRePRP1.2e0
- Outcome
- direct target of miR156j-3pe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsRePRP1.2 was confirmed as a direct target of miR156j-3p”
t0“rice”
Claim · model-written summary
OsRePRP1.2 positively regulated rice salt tolerance.
- Taxon
- riceOryza sativae0
- Gene
- OsRePRP1.2e0
- Outcome
- rice salt tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsRePRP1.2 positively regulated rice salt tolerance.”
Claim · model-written summary
STTM156j-3p plants showed enhanced hypersensitivity to exogenous ABA.
- Taxon
- riceOryza sativat0
- Material
- STTM156j-3pe0
- Treatment
- exogenous ABA treatmente0
- Outcome
- hypersensitivitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both STTM156j-3p and OsRePRP1.2 overexpression ( RePRP1.2 -ox) plants showed enhanced hypersensitivity to exogenous ABA treatment.”
t0“rice”
Claim · model-written summary
RePRP1.2 -ox plants showed enhanced hypersensitivity to exogenous ABA.
- Taxon
- riceOryza sativat0
- Material
- RePRP1.2 -oxe0
- Treatment
- exogenous ABA treatmente0
- Outcome
- hypersensitivitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both STTM156j-3p and OsRePRP1.2 overexpression ( RePRP1.2 -ox) plants showed enhanced hypersensitivity to exogenous ABA treatment.”
t0“rice”
Claim · model-written summary
Enhanced salt tolerance was associated with reduced hydrogen peroxide and malondialdehyde accumulation.
- Taxon
- riceOryza sativat0
- Material
- STTM156j-3pt1
- Outcome
- accumulation of hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA)e0
- Direction
- reducede0
- Qualifier
- enhanced salt tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced salt tolerance was associated with reduced accumulation of hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA)”
t0“rice”
t1“STTM156j-3p and RePRP1.2 -ox plants”
Claim · model-written summary
Enhanced salt tolerance was associated with elevated antioxidant enzyme activities.
- Taxon
- riceOryza sativat0
- Material
- STTM156j-3pt1
- Outcome
- antioxidant enzyme activitiese0
- Direction
- elevatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced salt tolerance was associated with reduced accumulation of hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA), along with elevated antioxidant enzyme activities.”
t0“rice”
t1“STTM156j-3p and RePRP1.2 -ox plants”
Claim · model-written summary
ABA signaling, biosynthesis, and ROS scavenging genes were up-regulated in STTM156j-3p plants.
- Taxon
- riceOryza sativat0
- Material
- STTM156j-3pe0
- Outcome
- genes involved in ABA signaling, ABA biosynthetic and ROS-scavenging system were up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the genes involved in ABA signaling, ABA biosynthetic and ROS-scavenging system were up-regulated in STTM156j-3p and RePRP1.2 -ox plants.”
t0“rice”
Claim · model-written summary
ABA signaling, biosynthesis, and ROS scavenging genes were up-regulated in RePRP1.2 -ox plants.
- Taxon
- riceOryza sativat0
- Material
- RePRP1.2 -oxe0
- Outcome
- genes involved in ABA signaling, ABA biosynthetic and ROS-scavenging system were up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the genes involved in ABA signaling, ABA biosynthetic and ROS-scavenging system were up-regulated in STTM156j-3p and RePRP1.2 -ox plants.”
t0“rice”
Claim · model-written summary
Suppression of miR156j-3p did not affect rice yield.
- Taxon
- riceOryza sativae0
- Treatment
- Suppression of miR156j-3pe0
- Outcome
- rice yielde0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of miR156j-3p or overexpression of OsRePRP1.2 did not affect rice yield.”
Claim · model-written summary
Overexpression of OsRePRP1.2 did not affect rice yield.
- Taxon
- riceOryza sativae0
- Treatment
- overexpression of OsRePRP1.2e0
- Outcome
- rice yielde0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppression of miR156j-3p or overexpression of OsRePRP1.2 did not affect rice yield.”
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