Abstract
Protoplasts of Page tangelo (Citrus reticulata Blanco×C. paradisi Macf.) cell suspension culture were electrically fused with mesophyll protoplasts isolated from trifoliate orange (Poncirus trifoliata (L.) Raf.). More than 150 plantlets regenerated after 4-5 months of culture. The regenerated plants were trifoliate with well developed root systems. Root_tip chromosome counting of more than 20 randomly selected plants revealed that they were all tetraploids (2n=4x=36). RAPD analysis of 7 randomly selected plants verified their hybridity. Inoculation of citrus Phytophthora parasitica Dastar toxin on leaves of somatic hybrids and both parental genotypes showed that Page tangelo was moderately susceptible, and trifoliate orange was highly resistant while the somatic hybrids were resistant. The potential of this somatic hybrid as rootstock is also discussed.
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Claims 7
Claim · model-written summary
More than 150 hybrid plantlets regenerated after 4-5 months.
- Taxon
- Citrus and Poncirus trifoliata
Citrus
Citrus trifoliatat0 - Outcome
- plantlets regeneratede0
- Value
- More than 150e0
- Qualifier
- after 4-5 months of culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“More than 150 plantlets regenerated after 4-5 months of culture.”
t0“Citrus and Poncirus trifoliata”
Claim · model-written summary
Regenerated hybrids had trifoliate leaves and well developed roots.
- Taxon
- Citrus and Poncirus trifoliataCitrusCitrus trifoliatat0
- Outcome
- trifoliate with well developed root systemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regenerated plants were trifoliate with well developed root systems.”
t0“Citrus and Poncirus trifoliata”
Claim · model-written summary
All chromosome-counted regenerated plants were tetraploid.
- Taxon
- Citrus and Poncirus trifoliataCitrusCitrus trifoliatat0
- Outcome
- all tetraploidse0
- Value
- 2n=4x=36e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root_tip chromosome counting of more than 20 randomly selected plants revealed that they were all tetraploids (2n=4x=36).”
t0“Citrus and Poncirus trifoliata”
Claim · model-written summary
RAPD analysis verified hybrid identity in seven plants.
- Taxon
- Citrus and Poncirus trifoliataCitrusCitrus trifoliatat0
- Outcome
- verified their hybriditye0
- Value
- 7 randomly selected plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RAPD analysis of 7 randomly selected plants verified their hybridity.”
t0“Citrus and Poncirus trifoliata”
Claim · model-written summary
Page tangelo showed moderate susceptibility to Phytophthora parasitica toxin.
- Taxon
- Page tangeloe0
- Treatment
- Inoculation of citrus Phytophthora parasitica Dastar toxine0
- Interactor
- Phytophthora parasitica
Phytophthora nicotianaee0pathogen - Outcome
- moderately susceptiblee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation of citrus Phytophthora parasitica Dastar toxin on leaves of somatic hybrids and both parental genotypes showed that Page tangelo was moderately susceptible, and trifoliate orange was highly resistant while the somatic hybrids were resistant.”
Claim · model-written summary
Trifoliate orange was highly resistant to Phytophthora parasitica toxin.
- Taxon
- trifoliate orangeCitrus trifoliatae0
- Treatment
- Inoculation of citrus Phytophthora parasitica Dastar toxine0
- Interactor
- Phytophthora parasiticaPhytophthora nicotianaee0pathogen
- Outcome
- highly resistante0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation of citrus Phytophthora parasitica Dastar toxin on leaves of somatic hybrids and both parental genotypes showed that Page tangelo was moderately susceptible, and trifoliate orange was highly resistant while the somatic hybrids were resistant.”
Claim · model-written summary
Somatic hybrids were resistant to Phytophthora parasitica toxin.
- Taxon
- Citrus and Poncirus trifoliataCitrusCitrus trifoliatat0
- Treatment
- Inoculation of citrus Phytophthora parasitica Dastar toxine0
- Interactor
- Phytophthora parasiticaPhytophthora nicotianaee0pathogen
- Outcome
- somatic hybrids were resistante0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation of citrus Phytophthora parasitica Dastar toxin on leaves of somatic hybrids and both parental genotypes showed that Page tangelo was moderately susceptible, and trifoliate orange was highly resistant while the somatic hybrids were resistant.”
t0“Citrus and Poncirus trifoliata”
The same questions in other papers
Phytophthora nicotianae · disease · CitrusResults agree
1 paper in the same direction as this paper
- positive
pathogen product · disease · CitrusPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
inoculation · disease · Citrus trifoliata (trifoliate orange)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
1 paper more in this group
See all 5 papers →inoculation · disease · CitrusPositive and negative results
10 papers in the same direction as this paper · 12 in the opposite direction
- no effect
- negative
- negative
19 papers more in this group
See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.