Abstract
Antioxidant components of avocado seed can be used to prevent browning caused by polyphenol oxidase (PPO). This research evaluated the inhibitory effect of avocado seed extract obtained through solid state fermentation on PPO and assessed the anti-browning performance of this extract on avocado, banana, and apple pulp in comparison to ascorbic acid and L-Cysteine. The Km and Vmax kinetic parameters of avocado PPO, using catechol as substrate, were 0.1627 g/L and 0.6577 Abs/min, respectively. The fermented extract completely inhibited the enzyme at 60 % v/v, likewise, the type of competitive inhibition of the fermented extract was determined by the Lineweaver-Burk method. Additionally, fermented extract effectively delayed browning in avocado, and banana at 0.5 % v/v and 1.0 % v/v in apple, the effect is linked to the presence of bioactive compounds as potential inhibitors capable of retarding the darkening. Mathematical modeling of enzyme inhibition was performed and it was found self-inhibition is present in most cases. In conclusion, fermented avocado seed extract is a potential product to protect other foodstuffs from oxidation in the food industry.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Avocado PPO had Km of 0.1627 g/L with catechol.
- Taxon
- avocado
Persea americanae0 - Outcome
- Kme0
- Value
- 0.1627 g/Le0
- Qualifier
- using catechol as substratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Km and Vmax kinetic parameters of avocado PPO, using catechol as substrate, were 0.1627 g/L and 0.6577 Abs/min, respectively.”
Claim · model-written summary
Avocado PPO had Vmax of 0.6577 Abs/min with catechol.
- Taxon
- avocadoPersea americanae0
- Outcome
- Vmaxe0
- Value
- 0.6577 Abs/mine0
- Qualifier
- using catechol as substratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Km and Vmax kinetic parameters of avocado PPO, using catechol as substrate, were 0.1627 g/L and 0.6577 Abs/min, respectively.”
Claim · model-written summary
Fermented extract completely inhibited avocado PPO at 60 % v/v.
- Taxon
- avocadoPersea americanat0
- Treatment
- fermented extracte0
- Outcome
- inhibited the enzymee0
- Direction
- completely inhibitede0
- Value
- 60 % v/ve0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fermented extract completely inhibited the enzyme at 60 % v/v”
t0“fermented avocado seed extract”
Claim · model-written summary
The fermented extract competitively inhibited avocado PPO.
- Taxon
- avocadoPersea americanat0
- Treatment
- fermented extracte0
- Outcome
- competitive inhibitione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the type of competitive inhibition of the fermented extract was determined by the Lineweaver-Burk method”
t0“fermented avocado seed extract”
Claim · model-written summary
Fermented extract delayed browning in avocado pulp at 0.5 % v/v.
- Taxon
- avocadoPersea americanae0
- Treatment
- fermented extracte0
- Outcome
- browninge0
- Direction
- delayede0
- Value
- 0.5 % v/ve0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fermented extract effectively delayed browning in avocado, and banana at 0.5 % v/v and 1.0 % v/v in apple”
Claim · model-written summary
Fermented extract delayed browning in banana pulp at 0.5 % v/v.
Not reportedComparator
Verbatim
e0“fermented extract effectively delayed browning in avocado, and banana at 0.5 % v/v and 1.0 % v/v in apple”
Claim · model-written summary
Fermented extract delayed browning in apple pulp at 1.0 % v/v.
- Taxon
- apple
Malus domesticae0 - Treatment
- fermented extracte0
- Outcome
- browninge0
- Direction
- delayede0
- Value
- 1.0 % v/ve0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fermented extract effectively delayed browning in avocado, and banana at 0.5 % v/v and 1.0 % v/v in apple”
Claim · model-written summary
Enzyme inhibition modeling found self-inhibition in most cases.
- Taxon
- avocadoPersea americanat0
- Outcome
- self-inhibitione0
- Qualifier
- in most casese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mathematical modeling of enzyme inhibition was performed and it was found self-inhibition is present in most cases.”
t0“fermented avocado seed extract”
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