Abstract
Leaf senescence is the final leaf developmental process that is regulated by both intracellular factors and environmental conditions. The mitogen-activated protein kinase(MAPK) signaling cascades have been shown to play important roles in regulating leaf senescence; however, the component(s) downstream of the MAPK cascades in regulating leaf senescence are not fully understood. Here we showed that the transcriptions of Zm MEK1, Zm SIMK1, and Zm MPK3 were induced during dark-induced maize leaf senescence.Furthermore, in-gel kinase analysis revealed the 42 k Da MAPK was activated. Zm MEK1 interacted with Zm SIMK1 in yeast and maize mesophyll protoplasts and Zm SIMK1 was activated by Zm MEK1 in vitro. Expression of a dominant negative mutant of Zm MEK1 in Arabidopsis transgenic plants induced salicylic acid(SA) accumulation and SA-dependent leaf senescence.Zm MEK1 interacted with Arabidopsis MPK4 in yeast and High-Im pact activated MPK4 in vitro. SA treatment accelerated darkinduced maize leaf senescence. Moreover, blockage of MAPK signaling increased endogenous SA accumulation in maize leaves. These findings suggest that Zm MEK1-Zm SIMK1 cascade and its modulating SA levels play important roles in regulating leaf senescence.
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Claims 11
Claim · model-written summary
Dark-induced maize leaf senescence induced transcription of Zm MEK1, Zm SIMK1 and Zm MPK3.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcriptions of Zm MEK1, Zm SIMK1, and Zm MPK3 were induced during dark-induced maize leaf senescence”
Claim · model-written summary
A 42 k Da MAPK was activated.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“dark-induced maize leaf senescence”
t0“leaf senescence”
e0“in-gel kinase analysis revealed the 42 k Da MAPK was activated”
Claim · model-written summary
Zm MEK1 interacted with Zm SIMK1 in yeast and maize mesophyll protoplasts.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zm MEK1 interacted with Zm SIMK1 in yeast and maize mesophyll protoplasts”
Claim · model-written summary
Zm MEK1 activated Zm SIMK1 in vitro.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“dark-induced maize leaf senescence”
e0“Zm SIMK1 was activated by Zm MEK1 in vitro”
Claim · model-written summary
Dominant negative Zm MEK1 expression increased salicylic acid accumulation in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Expression of a dominant negative mutant of Zm MEK1e0
- Gene
- Zm MEK1e0
- Outcome
- salicylic acid(SA) accumulatione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of a dominant negative mutant of Zm MEK1 in Arabidopsis transgenic plants induced salicylic acid(SA) accumulation and SA-dependent leaf senescence”
Claim · model-written summary
Dominant negative Zm MEK1 expression induced SA-dependent leaf senescence in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Expression of a dominant negative mutant of Zm MEK1e0
- Gene
- Zm MEK1e0
- Outcome
- SA-dependent leaf senescencee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of a dominant negative mutant of Zm MEK1 in Arabidopsis transgenic plants induced salicylic acid(SA) accumulation and SA-dependent leaf senescence”
Claim · model-written summary
Zm MEK1 interacted with Arabidopsis MPK4 in yeast.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- MPK4e0
- Outcome
- Zm MEK1 interacted with Arabidopsis MPK4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zm MEK1 interacted with Arabidopsis MPK4 in yeast”
Claim · model-written summary
Zm MEK1 activated MPK4 in vitro.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- MPK4e0
- Outcome
- activated MPK4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High-Im pact activated MPK4 in vitro”
e1“Arabidopsis MPK4 in yeast”
Claim · model-written summary
SA treatment accelerated dark-induced maize leaf senescence.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA treatment accelerated darkinduced maize leaf senescence”
Claim · model-written summary
Blocking MAPK signalling increased endogenous SA accumulation in maize leaves.
- Organ / stage
- leavese0
- Treatment
- blockage of MAPK signalinge0
- Outcome
- endogenous SA accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“blockage of MAPK signaling increased endogenous SA accumulation in maize leaves”
Claim · model-written summary
The authors propose that the Zm MEK1-Zm SIMK1 cascade regulates leaf senescence by modulating SA levels.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zm MEK1-Zm SIMK1 cascade and its modulating SA levels play important roles in regulating leaf senescence”
e1“dark-induced maize leaf senescence”
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