Paper · 2026 · Scientific Reports

Interactive effects of biochar and microbial biofertilizers on sandy soil fertility and cowpea yield in Egyptian agroecosystems

Doaa M. Khalifa; Heba M. Hewait; Alina-Stefania Stanciu; et al.

10
claims
28
verbatim quotes
1
citations
3
research areas
✓
Open access · cc-by-nc-nd

Abstract

Cowpea ( Vigna unguiculata ssp. unguiculata ) is a promising legume crop for arid and semi-arid regions due to its short growth duration, nutritional value, and drought tolerance. However, sandy soils in Egypt suffer from low fertility, weak aggregation, and poor water-holding capacity, which limit crop productivity. This study evaluated the combined effects of biochar and microbial inoculants ( Bacillus amyloliquefaciens and Saccharomyces cerevisiae ) on sandy soil fertility and cowpea productivity in Egypt. A two-season (2020–2021) field experiment was conducted in the Ismailia Governorate, Egypt, using cowpea cv. Dokki 126 in a randomized complete block design with 12 treatments (biochar, microbial inoculants, nitrogen fertilizer, and their combinations) and three replicates. The integrated application of biochar and microbial inoculants significantly increased total microbial counts by up to 65% and dehydrogenase activity by 42% compared to the control. Soil bulk density decreased by 18%, total porosity increased by 22%, and available soil N, P, and K contents increased by 45%, 52%, and 39%, respectively, leading to higher chlorophyll content (+ 28%), plant length (+ 21%), and seed yield (+ 33%) relative to the control. These findings indicate that integrating biochar with microbial inoculants significantly enhances sandy soil fertility and cowpea productivity, offering a sustainable pathway for soil restoration in arid ecosystems.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Integrated biochar and microbial inoculants changed cowpea-system total microbial counts by up to 65% relative to the control.

Treatment
integrated application of biochar and microbial inoculantse0
Outcome
total microbial countse0
Direction
increasede0
Value
up to 65%e0
Comparator
the controle0

Not reportedMaterial

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The integrated application of biochar and microbial inoculants significantly increased total microbial counts by up to 65% and dehydrogenase activity by 42% compared to the control.”

✓ located verbatim in the source · abstract, characters 790–971 · show in text ↑
t0

“Cowpea”

✓ located after whitespace/quote normalisation · title, characters 88–94 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Integrated biochar and microbial inoculants changed cowpea-system dehydrogenase activity by 42% relative to the control.

Treatment
integrated application of biochar and microbial inoculantse0
Outcome
dehydrogenase activitye0
Direction
increasede0
Value
42%e0
Comparator
the controle0

Not reportedMaterial

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The integrated application of biochar and microbial inoculants significantly increased total microbial counts by up to 65% and dehydrogenase activity by 42% compared to the control.”

✓ located verbatim in the source · abstract, characters 790–971 · show in text ↑
t0

“Cowpea”

✓ located after whitespace/quote normalisation · title, characters 88–94 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Integrated biochar and microbial inoculants changed cowpea-system Soil bulk density by 18% relative to the control.

Treatment
biochar and microbial inoculantse1
Outcome
Soil bulk densitye0
Direction
decreasede0
Value
18%e0
Comparator
the controle0

Not reportedMaterial

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Soil bulk density decreased by 18%, total porosity increased by 22%, and available soil N, P, and K contents increased by 45%, 52%, and 39%, respectively, leading to higher chlorophyll content (+ 28%), plant length (+ 21%), and seed yield (+ 33%) relative to the control.”

✓ located verbatim in the source · abstract, characters 972–1243 · show in text ↑
t0

“Cowpea”

✓ located after whitespace/quote normalisation · title, characters 88–94 · show in text ↑
e1

“integrated application of biochar and microbial inoculants”

✓ located verbatim in the source · abstract, characters 794–852 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Integrated biochar and microbial inoculants changed cowpea-system total porosity by 22% relative to the control.

Treatment
biochar and microbial inoculantse1
Outcome
total porositye0
Direction
increasede0
Value
22%e0
Comparator
the controle0

Not reportedMaterial

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Soil bulk density decreased by 18%, total porosity increased by 22%, and available soil N, P, and K contents increased by 45%, 52%, and 39%, respectively, leading to higher chlorophyll content (+ 28%), plant length (+ 21%), and seed yield (+ 33%) relative to the control.”

✓ located verbatim in the source · abstract, characters 972–1243 · show in text ↑
t0

“Cowpea”

✓ located after whitespace/quote normalisation · title, characters 88–94 · show in text ↑
e1

“integrated application of biochar and microbial inoculants”

✓ located verbatim in the source · abstract, characters 794–852 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Integrated biochar and microbial inoculants changed cowpea-system available soil N by 45% relative to the control.

Treatment
biochar and microbial inoculantse1
Outcome
available soil Ne0
Direction
increasede0
Value
45%e0
Comparator
the controle0

Not reportedMaterial

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Soil bulk density decreased by 18%, total porosity increased by 22%, and available soil N, P, and K contents increased by 45%, 52%, and 39%, respectively, leading to higher chlorophyll content (+ 28%), plant length (+ 21%), and seed yield (+ 33%) relative to the control.”

✓ located verbatim in the source · abstract, characters 972–1243 · show in text ↑
t0

“Cowpea”

✓ located after whitespace/quote normalisation · title, characters 88–94 · show in text ↑
e1

“integrated application of biochar and microbial inoculants”

✓ located verbatim in the source · abstract, characters 794–852 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Integrated biochar and microbial inoculants changed cowpea-system available soil P by 52% relative to the control.

Treatment
biochar and microbial inoculantse1
Outcome
available soil N, P, and K contentse0
Direction
increasede0
Value
52%e0
Comparator
the controle0

Not reportedMaterial

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Soil bulk density decreased by 18%, total porosity increased by 22%, and available soil N, P, and K contents increased by 45%, 52%, and 39%, respectively, leading to higher chlorophyll content (+ 28%), plant length (+ 21%), and seed yield (+ 33%) relative to the control.”

✓ located verbatim in the source · abstract, characters 972–1243 · show in text ↑
t0

“Cowpea”

✓ located after whitespace/quote normalisation · title, characters 88–94 · show in text ↑
e1

“integrated application of biochar and microbial inoculants”

✓ located verbatim in the source · abstract, characters 794–852 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Integrated biochar and microbial inoculants changed cowpea-system available soil K by 39% relative to the control.

Treatment
biochar and microbial inoculantse1
Outcome
available soil N, P, and K contentse0
Direction
increasede0
Value
39%e0
Comparator
the controle0

Not reportedMaterial

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Soil bulk density decreased by 18%, total porosity increased by 22%, and available soil N, P, and K contents increased by 45%, 52%, and 39%, respectively, leading to higher chlorophyll content (+ 28%), plant length (+ 21%), and seed yield (+ 33%) relative to the control.”

✓ located verbatim in the source · abstract, characters 972–1243 · show in text ↑
t0

“Cowpea”

✓ located after whitespace/quote normalisation · title, characters 88–94 · show in text ↑
e1

“integrated application of biochar and microbial inoculants”

✓ located verbatim in the source · abstract, characters 794–852 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Integrated biochar and microbial inoculants changed cowpea-system chlorophyll content by + 28% relative to the control.

Treatment
biochar and microbial inoculantse1
Outcome
chlorophyll contente0
Direction
highere0
Value
+ 28%e0
Comparator
the controle0

Not reportedMaterial

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Soil bulk density decreased by 18%, total porosity increased by 22%, and available soil N, P, and K contents increased by 45%, 52%, and 39%, respectively, leading to higher chlorophyll content (+ 28%), plant length (+ 21%), and seed yield (+ 33%) relative to the control.”

✓ located verbatim in the source · abstract, characters 972–1243 · show in text ↑
t0

“Cowpea”

✓ located after whitespace/quote normalisation · title, characters 88–94 · show in text ↑
e1

“integrated application of biochar and microbial inoculants”

✓ located verbatim in the source · abstract, characters 794–852 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Integrated biochar and microbial inoculants changed cowpea-system plant length by + 21% relative to the control.

Treatment
biochar and microbial inoculantse1
Outcome
plant lengthe0
Direction
highere0
Value
+ 21%e0
Comparator
the controle0

Not reportedMaterial

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Soil bulk density decreased by 18%, total porosity increased by 22%, and available soil N, P, and K contents increased by 45%, 52%, and 39%, respectively, leading to higher chlorophyll content (+ 28%), plant length (+ 21%), and seed yield (+ 33%) relative to the control.”

✓ located verbatim in the source · abstract, characters 972–1243 · show in text ↑
t0

“Cowpea”

✓ located after whitespace/quote normalisation · title, characters 88–94 · show in text ↑
e1

“integrated application of biochar and microbial inoculants”

✓ located verbatim in the source · abstract, characters 794–852 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Integrated biochar and microbial inoculants changed cowpea-system seed yield by + 33% relative to the control.

Treatment
biochar and microbial inoculantse1
Outcome
seed yielde0
Direction
highere0
Value
+ 33%e0
Comparator
the controle0

Not reportedMaterial

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Soil bulk density decreased by 18%, total porosity increased by 22%, and available soil N, P, and K contents increased by 45%, 52%, and 39%, respectively, leading to higher chlorophyll content (+ 28%), plant length (+ 21%), and seed yield (+ 33%) relative to the control.”

✓ located verbatim in the source · abstract, characters 972–1243 · show in text ↑
t0

“Cowpea”

✓ located after whitespace/quote normalisation · title, characters 88–94 · show in text ↑
e1

“integrated application of biochar and microbial inoculants”

✓ located verbatim in the source · abstract, characters 794–852 · show in text ↑

inoculation · yield · Vigna unguiculata (cowpea)Results differ

116 papers: 99 positive, 17 negative or no effect

98 papers in the same direction as this paper · 16 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. no effect

112 papers more in this group

See all 116 papers →

biochar · soil: biology · Vigna unguiculata (cowpea)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive
See all 4 papers →

inoculation · physiology: pigments · Vigna unguiculata (cowpea)Positive and negative results

9 papers: 8 positive, 1 negative or no effect

7 papers in the same direction as this paper · 1 reporting no difference from an alternative

  1. no effect
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

biochar · growth · Vigna unguiculata (cowpea)Positive and negative results

15 papers: 13 positive, 2 negative or no effect

12 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. no effect
  3. positive

11 papers more in this group

See all 15 papers →

biochar · yield · Vigna unguiculata (cowpea)Positive and negative results

22 papers: 18 positive, 4 negative or no effect

17 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. negative
  3. no effect

18 papers more in this group

See all 22 papers →

inoculation · nutrient: content · Vigna unguiculata (cowpea)Positive and negative results

43 papers: 41 positive, 2 negative or no effect

40 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

39 papers more in this group

See all 43 papers →

inoculation · growth · Vigna unguiculata (cowpea)Positive and negative results

102 papers: 92 positive, 10 negative or no effect

91 papers in the same direction as this paper · 8 in the opposite direction · 2 reporting no difference from an alternative

  1. no effect
  2. negative
  3. no effect

98 papers more in this group

See all 102 papers →

biochar · physiology: pigments · Vigna unguiculata (cowpea)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

8 of 10 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.