Intervention effectmodel-written summary

HIGS targeting SsGAP1 reduced fungal virulence in Nicotiana and Arabidopsis.

A claim from RAS signalling genes can be used as host‐induced gene silencing targets to control fungal diseases caused by Sclerotinia sclerotiorum and Botrytis cinerea · 2023

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

HIGS targeting SsGAP1 reduced fungal virulence in Nicotiana and Arabidopsis.

Taxon
Nicotiana benthamiana and Arabidopsis thalianaArabidopsis thalianaNicotiana benthamianae0
Treatment
HIGS constructs targeting SsGAP1e0
Gene
SsGAP1e0
Outcome
virulencee0
Direction
reducede0

Not reportedMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arabidopsis thaliana
Arabidopsis thaliana · TaxonOriginal uploader was Brona at en.wikipedia . User:Roepers at nl.wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗
Nicotiana benthamiana
Nicotiana benthamiana · TaxonCharles Andres · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“when we introduced HIGS constructs targeting SsGAP1, SsRAS1 and SsRAS2 in Nicotiana benthamiana and Arabidopsis thaliana, we observed reduced virulence”

✓ located verbatim in the source · abstract, characters 1186–1337

Title

RAS signalling genes can be used as host‐induced gene silencing targets to control fungal diseases caused by Sclerotinia sclerotiorum and Botrytis cinerea

Yan Xu; Jinyi Tan; Junxing Lu; et al. Plant Biotechnology Journal 2023. doi:10.1111/pbi.14184

Other claims from this paper (7)

Record rev-c4c4e6bea26061a12e49b2fb6ecdbd4fd7acbe83f580b0e2c9266b26bc12d1b4 · schema claims-v0.9 · position 5 in the model’s answer

loss of function · disease · Arabidopsis thaliana (thale cress)Positive and negative results

82 papers: 31 positive, 51 negative or no effect

30 papers in the same direction as this claim · 51 in the opposite direction

  1. negative
    SOS2 mutations increased sensitivity to growth inhibition under high sodium and low potassium.

    The Arabidopsis thaliana SOS2 gene encodes a protein kinase that is required for salt tolerance · 2000

    Opposite direction

  2. negative
    Mutation in LysM RLK1 increased susceptibility to fungal pathogens.

    A LysM Receptor-Like Kinase Plays a Critical Role in Chitin Signaling and Fungal Resistance in Arabidopsis · 2008

    Opposite direction

  3. negative
    LHCB down-regulation or disruption decreases drought tolerance.

    Light-harvesting chlorophyll a/b-binding proteins are required for stomatal response to abscisic acid in Arabidopsis · 2011

    Opposite direction

  4. negative
    GH3.5 insertion mutations partially compromised systemic acquired resistance.

    Dual Regulation Role of GH3.5 in Salicylic Acid and Auxin Signaling during Arabidopsis-Pseudomonas syringae Interaction · 2007

    Opposite direction

  5. no effect
    NPQ1 mutations do not affect any aspect of blue-light growth inhibition within the first 120 min.

    Unexpected roles for cryptochrome 2 and phototropin revealed by high‐resolution analysis of blue light‐mediated hypocotyl growth inhibition · 2001

    Opposite direction

  6. negative
    MYB15 mutation increased susceptibility to Pseudomonas pathogens.

    Coordination of microbe–host homeostasis by crosstalk with plant innate immunity · 2021

    Opposite direction

  7. negative
    Double disruption of CPK17 and CPK34 reduced pollen transmission efficiency approximately 350-fold.

    Calcium‐dependent protein kinases regulate polarized tip growth in pollen tubes · 2009

    Opposite direction

  8. negative
    Loss of bHLH115 caused strong iron deficiency symptoms.

    bHLH transcription factor bHLH115 regulates iron homeostasis in Arabidopsis thaliana · 2017

    Opposite direction

73 papers more in this group

See all 82 papers →

loss of function · disease · Nicotiana benthamianaPositive and negative results

143 papers: 50 positive, 93 negative or no effect

49 papers in the same direction as this claim · 93 in the opposite direction

  1. negative
    Deleting the V. dahliae glucosyltransferase gene impaired full virulence on Nicotiana benthamiana.

    Comparative Genomics Yields Insights into Niche Adaptation of Plant Vascular Wilt Pathogens · 2011

    Opposite direction

  2. negative
    Antisense SNF1 expression enhances susceptibility in Nicotiana benthamiana.

    Geminivirus AL2 and L2 Proteins Interact with and Inactivate SNF1 Kinase · 2003 · compared with AL2 and L2 transgenes

    Opposite direction

  3. negative
    Silencing Nt CMPG1 reduced the hypersensitive response in N. benthamiana after Cf-9/Avr9 elicitation.

    The U-Box Protein CMPG1 Is Required for Efficient Activation of Defense Mechanisms Triggered by Multiple Resistance Genes in Tobacco and Tomato · 2006

    Opposite direction

  4. negative
    Silencing three genes compromised Cf-mediated hypersensitive response in N. benthamiana.

    Functional Analysis ofAvr9/Cf-9 Rapidly ElicitedGenes Identifies a Protein Kinase, ACIK1, That Is Essential for Full Cf-9–Dependent Disease Resistance in Tomato · 2004

    Opposite direction

  5. negative
    SGT1 silencing increased PlAMV accumulation.

    Viral-Induced Systemic Necrosis in Plants Involves Both Programmed Cell Death and the Inhibition of Viral Multiplication, Which Are Regulated by Independent Pathways · 2010

    Opposite direction

  6. negative
    Silencing δOAT and ProDH in N. benthamiana delayed the hypersensitive response.

    Ornithine‐delta‐aminotransferase and proline dehydrogenase genes play a role in non‐host disease resistance by regulating pyrroline‐5‐carboxylate metabolism‐induced hypersensitive response · 2012

    Opposite direction

  7. negative
    Silencing NbSKP1s enhances CLCuMuV genomic DNA accumulation.

    CLCuMuB βC1 Subverts Ubiquitination by Interacting with NbSKP1s to Enhance Geminivirus Infection in Nicotiana benthamiana · 2016

    Opposite direction

  8. negative
    NbAXS1 silencing caused growth arrest and leaf yellowing.

    Depletion of UDP-d-apiose/UDP-d-xylose Synthases Results in Rhamnogalacturonan-II Deficiency, Cell Wall Thickening, and Cell Death in Higher Plants · 2006

    Opposite direction

134 papers more in this group

See all 143 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.