Search claims · PlantClaims

Observationobservedoriginal resultmeasured

Claim · model-written summary

Single stranded templates underwent multiple replication rounds.

Taxon: peaLathyrus oleraceust0
Outcome: single-stranded DNA templates undergo more than one round of replicatione0

Verbatim

e0
“Both strands of template DNA are synthesized and single-stranded DNA templates undergo more than one round of replication.”
✓ located verbatim in the source · abstract, characters 306–428

t0
“pea”
✓ located verbatim in the source · title, characters 53–56

Title

Characterisation and mode of in vitro replication of pea chloroplast OriA sequences

Malireddy K. REDDY; Nirupam Roy CHOUDHURY; Dhirendra KUMAR; et al. European Journal of Biochemistry 1994. doi:10.1111/j.1432-1033.1994.tb18697.x

Other claims from this paper (7)

Method / resource The pea chloroplast system replicated recombinant origin containing DNA.
Observation Polymerization was fast and insensitive to chain terminators.
Observation Both template strands were synthesized.
Observation Either origin yielded sigma replication intermediates.
Observation In vitro initiation mapped near displacement loop regions.
Observation OriA in vitro initiation was chloroplast DNA specific but differed in replication mode from in vivo.
Observation Template containing both origins replicated in theta mode in vitro.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Shorter templates with HCPro enabled effective trans-replication in plant cells.

Taxon: Nicotiana benthamianaNicotiana benthamianae1
Treatment: addition of helper component proteinase (HCPro)e0
Interactor: Potato virus XPotato virus Xt0pathogen
Outcome: effective trans-replication of the templates in plant cellse0
Value: shorter templatese0

Verbatim

e0
“Further optimization of the system with shorter templates and addition of helper component proteinase (HCPro), a potyviral silencing suppressor, led to effective trans-replication of the templates in plant cells.”
✓ located verbatim in the source · abstract, characters 1275–1487

e1
“Nicotiana benthamiana leaves”
✓ located verbatim in the source · abstract, characters 1001–1029

t0
“Potato Virus X”
✓ located verbatim in the source · title, characters 21–35

Title

Trans-replication of Potato Virus X in planta

Pinky Dutta Työväentutkimus Vuosikirja 2018

Other claims from this paper (2)

Method / resource Longer templates did not replicate at four or six days when replicase was supplied in trans.
Observation Replication sites were scattered across the leaf lamina.

Mechanismhypothesisoriginal resultbasis n/a

Claim · model-written summary

The authors propose that replication templates in the centromere originate from multiple CENH3-nucleosome complexes bound to CentGm sequences.

Taxon: soybeanGlycine maxt0
Gene: CENH3e0
Outcome: replication templatese0

Verbatim

t0
“soybean centromeres”
✓ located verbatim in the source · title, characters 48–67

e0
“We propose a model in which replication templates within the centromere region originate from multiple CENH3-nucleosome complexes bound to CentGm sequences”
✓ located verbatim in the source · abstract, characters 1491–1646

Title

Genetic diversity and architectural dynamics of soybean centromeres

Yicheng Huang; Enlai Guan; Shipeng Song; et al. Genome biology 2026. doi:10.1186/s13059-025-03924-9

Other claims from this paper (6)

Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Distinct BARE transcript subfamilies may separately supply proteins and replication templates.

Gene: BAREe0
Outcome: providing the proteins and replication templatese0
Qualifier: may play independent rolese0

Not reportedTaxon

Verbatim

e0
“The data suggest that distinct subfamilies of transcripts may play independent roles in providing the proteins and replication templates for the BARE retrotransposon life cycle.”
✓ located verbatim in the source · abstract, characters 1462–1639

Title

BARE retrotransposons produce multiple groups of rarely polyadenylated transcripts from two differentially regulated promoters

Wei Chang; Alan H. Schulman The Plant Journal 2008. doi:10.1111/j.1365-313x.2008.03572.x

Other claims from this paper (10)

Observation The BARE family has more than 10(4) copies in the barley genome.
Observation BARE1 elements are transcribed, translated, and form virus-like particles.
Observation BARE2 elements cannot produce the GAG capsid protein.
Observation The two BARE LTR promoters vary independently in activity among tissues.
Observation TATA1 was almost inactive in embryos.
Observation BARE transcription appeared less tightly regulated in callus than other tissues.
Observation LTR deletions revealed strong positive and negative regulatory elements.
Observation The promoters generated transcript groups differing in ends, sequences, and polyadenylation.
Observation Some transcript groups lack common end structures needed for template switching.
Observation Only about 15% of BARE transcripts are polyadenylated.

Mechanismobservedreview summarybasis n/a

Claim · model-written summary

Host factors participate in assembling and activating viral RNA replication complexes and selecting templates.

Taxon: Plantt0
Outcome: assembling the viral RNA replication complex, selecting and recruiting viral RNA replication templates, activating the viral complex for RNA synthesise0
Qualifier: host factorse0

Not reportedDirection

Verbatim

e0
“Recent data show that host factors play important roles in assembling the viral RNA replication complex, selecting and recruiting viral RNA replication templates, activating the viral complex for RNA synthesis, and the other steps.”
✓ located verbatim in the source · abstract, characters 716–947

t0
“Plant RNA Viruses”
✓ located verbatim in the source · title, characters 54–71

Title

The Importance of Host Factors for the Replication of Plant RNA Viruses

Mi-Ri Park; Kook-Hyung Kim Research in Plant Disease 2005. doi:10.5423/rpd.2005.11.2.098

Other claims from this paper (4)

Mechanismauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Mutations from viral polymerase error arise at a constant rate, but most new mutants are sequestered in virions and are not replication templates.

Taxon: wheat, corn, and barleyHordeum vulgare Triticum aestivumZea mays subsp. mayse3
Outcome: mutations arising by viral polymerase errore4
Qualifier: most newly generated mutants are sequestered in virions and do not serve as replication templatese4

Verbatim

e3
“to establish three lineages each in wheat, corn, and barley”
✓ located verbatim in the source · abstract, characters 462–521

e4
“mutations arising by viral polymerase error are generated at a constant rate but that most newly generated mutants are sequestered in virions and do not serve as replication templates”
✓ located verbatim in the source · abstract, characters 1384–1567

Title

Structure and Temporal Dynamics of Populations within Wheat Streak Mosaic Virus Isolates

Jeffrey S. Hall; Roy French; T. Jack Morris; et al. Journal of Virology 2001. doi:10.1128/jvi.75.21.10231-10243.2001

Other claims from this paper (9)

Mechanismhypothesisoriginal resultbasis n/a

Claim · model-written summary

Discontinuous RNA replication, with the replicase switching templates, may generate RNA diversity.

Taxon: local lesion hoste0
Outcome: RNA diversitye5
Qualifier: discontinuous RNA replicatione5

Verbatim

e0
“Pairs of BMV RNA3 variants carrying marker mutations at different locations were coinoculated on a local lesion host, and the progeny RNA3 in a large number of lesions was analyzed”
✓ located verbatim in the source · abstract, characters 179–359

e5
“Our results suggest discontinuous RNA replication, with the replicase changing among the homologous RNA templates and generating RNA diversity”
✓ located verbatim in the source · abstract, characters 993–1135

Title

Frequent Homologous Recombination Events between Molecules of One RNA Component in a Multipartite RNA Virus

A. Bruyere; M. Wantroba; S. Flasinski; et al. Journal of Virology 2000. doi:10.1128/jvi.74.9.4214-4219.2000

Other claims from this paper (6)

Observationobservedoriginal resultmeasured

Claim · model-written summary

ER-associated enzyme had viral RNAs as endogenous templates.

Taxon: tobacco plantsNicotiana tabacumt0
Organ / stage: ERe0
Outcome: viral RNAs as endogenous templatese0

Verbatim

e0
“The ER-associated enzyme had viral RNAs as endogenous templates”
✓ located verbatim in the source · abstract, characters 567–630

t0
“tobacco plants”
✓ located verbatim in the source · title, characters 74–88

Title

Characterization and localization of the viral RNA replication complex in tobacco plants infected with cucumber mosaic virus.

Yoichi TAKANAMI; Naoto NITTA; Susumu KUBO Japanese Journal of Phytopathology 1989. doi:10.3186/jjphytopath.55.635

Other claims from this paper (9)

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Recombinants pCP 12-7 and pCB 1-12 were highly active in DNA synthesis as templates.

Taxon: peaLathyrus oleraceuse0
Material: pCP 12-7 and pCB 1-12e0
Outcome: DNA synthesise0
Direction: highly activee0

Verbatim

t0
“pea”
✓ located verbatim in the source · title, characters 39–42

e0
“The recombinants pCP 12-7 and pCB 1-12 were found to be highly active in DNA synthesis when used as templates in a partially purified replication system from pea chloroplasts.”
✓ located verbatim in the source · abstract, characters 1255–1430

Title

Localization of replication origins in pea chloroplast DNA.

R Meeker; B Nielsen; K K Tewari Molecular and Cellular Biology 1988. doi:10.1128/mcb.8.3.1216

Other claims from this paper (10)

Observation Both pea chloroplast replication origins were in the 44-kbp SalI A fragment.
Observation The first D-loop was 9.0 kbp from the closest SalI site.
Observation The first D-loop averaged about 0.7 kbp.
Observation The second D-loop extended from 14.2 to about 15.5 kbp from the same restriction site.
Observation The second D-loop was about 1.3 kbp in size.
Observation One D-loop mapped between the 16S and 23S rRNA genes.
Observation The second D-loop mapped downstream of the 23S rRNA gene.
Observation The D-loops differed in base composition, with the one nearest a SalI site denaturing more readily.
Observation Either D-loop recombinant supported full-length template DNA synthesis.
Observation Recombinants from other pea chloroplast regions showed no significant in vitro DNA synthesis.

Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Sense interfering transcripts served as templates for minus-strand synthesis.

Taxon: barleyHordeum vulgaree1
Outcome: templates for (-) strand synthesise0
Qualifier: sense interfering transcriptse0

Verbatim

e0
“The appearance of complementary (-) strands indicated that sense interfering transcripts could serve as templates for (-) strand synthesis”
✓ located verbatim in the source · abstract, characters 687–825

e1
“barley protoplasts”
✓ located verbatim in the source · abstract, characters 355–373

Title

Interference with brome mosaic virus replication by targeting the minus strand promoter

C. C. Huntley; T. C. Hall Journal of General Virology 1993. doi:10.1099/0022-1317-74-11-2445

Other claims from this paper (7)

Intervention effect Sense or antisense promoter transcripts reduced viral progeny RNA accumulation by more than 90% with RNAs 1 and 2.
Intervention effect With RNA 3 present, promoter transcripts reduced viral progeny accumulation by 60 to 80%.
Association Trans interference depended on transcript concentration.
Intervention effect Trans interference reduced positive and negative strand viral progeny similarly.
Mechanism Deletion mutants indicated template activity partly mediated interference.
Method / resource The protoplast assay was reproducible enough for rapid comparison of resistance strategies.
Recommendation The authors suggested targeting viral minus-strand promoters for engineered plant virus resistance.

“replication templates”

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