Search claims · PlantClaims

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

ELD1 is essential for rice embryo survival.

Taxon: riceOryza sativae0
Gene: ELD1e0
Outcome: rice embryo survivale0

Verbatim

t0
“in rice”
✓ located verbatim in the source · title, characters 111–118

e0
“ELD1, a CCHC-type zinc finger protein that is essential for rice embryo survival”
✓ located verbatim in the source · abstract, characters 232–312

Title

ELD1 mediates photoperiodic flowering via OsCCA1 alternative splicing and interacts with phytochrome signaling in rice

Liang Cai; Benyuan Hao; Zhuang Xu; et al. Nature Communications 2025. doi:10.1038/s41467-025-60839-6

Other claims from this paper (4)

Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Extra C chromosomes may have facilitated gamete success or embryo survival.

Taxon: Brassica rapaBrassica rapae1
Outcome: gamete success and/or embryo survivale0
Qualifier: extra C‐chromosomese0

Verbatim

e0
“suggesting that extra C‐chromosomes facilitated gamete success and/or embryo survival.”
✓ located verbatim in the source · abstract, characters 783–869

e1
“diploid wild Brassica rapa”
✓ located verbatim in the source · abstract, characters 31–57

Title

Performance of aneuploid backcross hybrids between the crop Brassica napus and its wild relative B. rapa

T. J. de Jong; K. Escobedo Quevedo; C. A. M. van der Veen‐van Wijk; et al. Plant Biology 2017. doi:10.1111/plb.12629

Other claims from this paper (6)

Observation BC1 average DNA value was similar to parental values.
Observation F2 average DNA value was 13% higher than F1.
Observation Under optimal and drought conditions, BC1 hybrid growth and survival were similar to B. rapa.
Association Growth and DNA value were not significantly correlated.
Association Survival and DNA value were not significantly correlated.
Observation Aneuploid plants were not inferior in the growth room and may persist in nature.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The protocol produced 20 transgenic clones per 0.5g inoculated tissue with 80% embryo survival in Kent mango.

Taxon: mangoMangifera indicat0
Material: Kente1
Outcome: transgenic clonese0
Value: Twenty transgenic clones per 0.5ge0
Qualifier: 80% embryo survivale0

Verbatim

e0
“Twenty transgenic clones per 0.5g of inoculated tissues with 80% embryo survival were produced”
✓ located verbatim in the source · abstract, characters 1338–1432

e1
“transgenic somatic embryos and plants of the Kent variety”
✓ located verbatim in the source · abstract, characters 1259–1316

t0
“mango”
✓ located verbatim in the source · title, characters 51–56

Title

Insertion of Agrobacterium rhizogenes rolB Gene in mango

Marleny Chavarri; Ariadne Vegas García; Asia Y. Zambrano; et al. Redalyc (Universidad Autónoma del Estado de México) 2010

Other claims from this paper (5)

Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Transformation efficiency and embryo survival were similar across the three strains.

Taxon: Typha domingensisTypha domingensise1
Outcome: efficiency of transformation and survival of the oblong and scutellar embryose0
Direction: similare0
Comparator: three bacterial strainse0

Verbatim

e0
“The efficiency of transformation and survival of the oblong and scutellar embryos were similar among the three bacterial strains.”
✓ located verbatim in the source · abstract, characters 977–1106

e1
“Typha domingensis seedlings”
✓ located verbatim in the source · abstract, characters 83–110

Title

Induction of Hairy Roots on Somatic Embryos of Rhizoclones from Typha domingensis Seedlings

Guadalupe Hernández-Piedra; Violeta Ruiz-Carrera; Alberto J. Sánchez; et al. Plants 2020. doi:10.3390/plants9121679

Other claims from this paper (4)

Method / resource A protocol for hairy-root induction on Typha domingensis somatic embryos was established.
Intervention effect Transformed embryos developed hairy roots, unlike nontransformed embryos.
Association Hairy-root number, length and density depended on bacterial strain.
Observation K599 was the most effective bacterial strain for hairy-root induction.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Gentamicin had the strongest inhibitory effect on embryo survival.

Taxon: Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
Treatment: Gte0
Outcome: survival of anther derived embryose0
Direction: strongest inhibitory effecte0

Not reportedComparator

Verbatim

t0
“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
✓ located verbatim in the source · title, characters 94–143

e0
“Gt shows the strongest inhibitory effect on the survival of anther derived embryos”
✓ located verbatim in the source · abstract, characters 659–741

Title

Effect of antibiotics on Agrobacterium-mediated transformation from anther derived embryos of Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’

Lihua Lin; Li Junqiang; Hua Zong; et al. The Journal of Horticultural Science and Biotechnology 2020. doi:10.1080/14620316.2020.1814876

Other claims from this paper (13)

Method / resource A stable transformation protocol was established for anther-derived loquat embryos.
Intervention effect Strain EHA 52 was completely insensitive to carbenicillin at every tested concentration.
Intervention effect Ampicillin was poisonous to both embryos and strain EHA 52.
Intervention effect Cephalothin was poisonous to both embryos and strain EHA 52.
Intervention effect Gentamicin was poisonous to both embryos and strain EHA 52.
Intervention effect Ampicillin gave the highest embryo survival rate, 42.00%.
Recommendation The authors considered Carb unsuitable for further transformation work.
Recommendation The authors considered Gt unsuitable for further transformation work.
Recommendation The authors suggested 800 mg·L−1 Amp with 100 mg·L−1 Kan for selecting transgenic secondary embryos.
Observation PCR indicated secondary-embryo transformation efficiency up to 22.00%.
Observation Transformed secondary embryo cells were smaller than cells of on-transformants.
Observation Cell division was more rapid in transformed than non-transformed secondary embryos.
Observation Nine KanR plantlets from two anther-derived embryos had the expected PCR fragment.

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ampicillin gave the highest embryo survival rate, 42.00%.

Taxon: Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’Eriobotrya japonicat0
Treatment: Ampe0
Outcome: survival ratee0
Direction: higheste0
Value: 42.00%e0

Not reportedComparator

Verbatim

t0
“Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’”
✓ located verbatim in the source · title, characters 94–143

e0
“Amp is the optimal antibiotic for the highest survival rate (42.00%) of the anther derived embryos”
✓ located verbatim in the source · abstract, characters 559–657

Title

Effect of antibiotics on Agrobacterium-mediated transformation from anther derived embryos of Eriobotrya japonica (Thunb.) Lindl. cv.‘Dawuxing’

Lihua Lin; Li Junqiang; Hua Zong; et al. The Journal of Horticultural Science and Biotechnology 2020. doi:10.1080/14620316.2020.1814876

Other claims from this paper (13)

Method / resource A stable transformation protocol was established for anther-derived loquat embryos.
Intervention effect Strain EHA 52 was completely insensitive to carbenicillin at every tested concentration.
Intervention effect Ampicillin was poisonous to both embryos and strain EHA 52.
Intervention effect Cephalothin was poisonous to both embryos and strain EHA 52.
Intervention effect Gentamicin was poisonous to both embryos and strain EHA 52.
Intervention effect Gentamicin had the strongest inhibitory effect on embryo survival.
Recommendation The authors considered Carb unsuitable for further transformation work.
Recommendation The authors considered Gt unsuitable for further transformation work.
Recommendation The authors suggested 800 mg·L−1 Amp with 100 mg·L−1 Kan for selecting transgenic secondary embryos.
Observation PCR indicated secondary-embryo transformation efficiency up to 22.00%.
Observation Transformed secondary embryo cells were smaller than cells of on-transformants.
Observation Cell division was more rapid in transformed than non-transformed secondary embryos.
Observation Nine KanR plantlets from two anther-derived embryos had the expected PCR fragment.

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Coconut water, vigorous immature embryos and removing excess bacteria significantly improved embryo survival.

Taxon: sorghumSorghume1
Organ / stage: sorghum embryose0
Treatment: The addition of coconut water to the co-cultivation mediume0
Outcome: survival rate of sorghum embryose0
Direction: improvede0

Not reportedComparator

Verbatim

e0
“The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos”
✓ located verbatim in the source · abstract, characters 783–995

e1
“transformation of sorghum”
✓ located verbatim in the source · abstract, characters 86–111

Title

Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency

Carlos Henrique S. Carvalho; Usha B. Zehr; Nilupa Gunaratna; et al. Genetics and Molecular Biology 2004. doi:10.1590/s1415-47572004000200022

Other claims from this paper (7)

Method / resource Agrobacterium mediated sorghum transformation was feasible.
Association Embryo sensitivity, donor growth conditions, explant type and cocultivation medium most influenced transformation.
Observation Explants developed necrosis after cocultivation.
Association Immature embryos were sensitive to Agrobacterium infection, and embryo death limited transformation efficiency.
Method / resource Hygromycin phosphotransferase was a good selectable marker.
Observation GUS activity was low in most transgenic tissues and very high in immature inflorescences.
Method / resource Overall transformation efficiency remained low.

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The nucellar embryo transformation procedure generated modified black pepper with 99% survival.

Taxon: black peppere0
Material: Sreekara and Karimundae0
Organ / stage: nucellar apomixis-derived embryose0
Outcome: survival ratee0
Value: 99%e0

Verbatim

e0
“generating genetically modified black pepper plants using nucellar apomixis-derived embryos of the varieties Sreekara and Karimunda, with a 99% survival rate”
✓ located verbatim in the source · abstract, characters 423–580

Title

Reducing recalcitrance of black pepper to Agrobacterium -mediated transformation: an efficient way through nucellar apomixis to establish transgenic and genome-edited plants at high frequency and scale-up through bioreactor

Shina Sasi; Saranya Krishnan; Martin Kottackal; et al. Horticulture Research 2026. doi:10.1093/hr/uhag067

Other claims from this paper (11)

Method / resource Agrobacterium tumefaciens transformed black pepper efficiently.
Method / resource Agrobacterium rhizogenes transformed black pepper efficiently.
Method / resource AGL1 carrying mgfp achieved over 90% transformation frequency under the stated procedure.
Association Sugar type determined embryonal taproot development and soil establishment.
Intervention effect Glucose supplemented medium yielded plantlets with developed roots.
Observation Plantlets from glucose supplemented medium had high PnPIN2 expression.
Method / resource Transgenic plantlets from glucose supplemented liquid medium survived ex vitro at 99%.
Method / resource CRISPR/Cas9 editing of Pds reached 89% efficiency.
Method / resource Composite black pepper plants were generated at over 60% efficacy.
Method / resource Black pepper agroinfiltration was useful for disease resistance screening.
Method / resource Temporary immersion bioreactors scaled production of modified and unmodified black pepper plants.

Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors conclude that spermidine synthase plays a critical role in plant embryo development, based on genetic evidence.

Taxon: ArabidopsisArabidopsist0
Gene: spermidine synthasee0
Outcome: embryo developmente0

Verbatim

e0
“These data provide the first genetic evidence indicating a critical role of the spermidine synthase in plant embryo development”
✓ located verbatim in the source · abstract, characters 1009–1136

t0
“Arabidopsis”
✓ located verbatim in the source · title, characters 56–67

Title

Spermidine Synthase Genes Are Essential for Survival of Arabidopsis

Akihiro Imai; Takashi Matsuyama; Yoshie Hanzawa; et al. PLANT PHYSIOLOGY 2004. doi:10.1104/pp.104.041699

Other claims from this paper (6)

Observationobservedoriginal resultmeasured

Claim · model-written summary

Agrobacteria were detected in maize embryo tissue as early as 24 h after inoculation.

Taxon: maizeZea mayst0
Organ / stage: maize embryo tissuese0
Interactor: agrobacteriae1other
Outcome: detected in maize embryo tissuese0
Value: 24 he0

Verbatim

e0
“were detected in maize embryo tissues as aerly as 24 h after inoculation”
✓ located verbatim in the source · abstract, characters 507–579

e1
“agrobacteria could spread”
✓ located verbatim in the source · abstract, characters 433–458

t0
“maize pistil filaments”
✓ located verbatim in the source · title, characters 76–98

Title

Analysis of proliferation and survival of agrobacteria after inoculation of maize pistil filaments

I. V. Volokhina; V. A. Velikov; E. M. Moiseeva; et al. Microbiology 2016. doi:10.1134/s0026261716010148

Other claims from this paper (2)

“embryo survival”

Claim types

Direction of the findings

Over time

Claim type

Research area

Polarity

Statistics